Figure 7
From: Live cell flattening — traditional and novel approaches
Characterization of the single layer microfluidic device. (A) The channel was filled with 100 μM fluorescein, and the intensity in the middle of the device was recorded. Between t = 85 s (line 1) and t = 685 s (line 2), the syringe pump withdrew 250 μl/h of the fluorescein through the device, leading to a drop in the channel height. This flow rate was used to flatten cAR1-GFP cells. Confocal x-y scans are shown for a cAR1-GFP cell (B) before and (C) during flattening. (D, E) show the corresponding x-z sections. The blue lines in (B, C) show the y-values for the sections (D, E), and the blue lines in (D, E) show the z-value of images (B, C). Scale bar: 10 μm.