Figure 2
From: Live cell flattening — traditional and novel approaches
Single layer microfluidic device. (A) Assembled single layer device with connected tubing, filled with ink. (B) Geometry of the chrome mask used to create the single layer device. The height of this channel was 14 μm. Two rows of pillars (red spots) support the ceiling. In (C), the principle of operation is shown. The inlet of the collapsing device is connected to a reservoir, filled with PB. A syringe pump removes the fluid at a constant rate (indicated by the gray arrows) and thus decreases the pressure within the microfluidic channel. This leads to a collapse of the ceiling and therefore to flattening of D. discoideum cells located in the channel.