Figure 6
From: Self-organizing actin waves that simulate phagocytic cup structures
Arrangement of actin-associated proteins in phagocytic cups. During the uptake of yeast particles, cells were imaged to localize GFP-tagged myosin-IB, Arp2/3, or coronin (green) in combination with filamentous actin (red). Centers of the particles are indicated by asterisks. A, bright-field image of a late phagocytosis stage showing a particle being engulfed. B, double-fluorescence recording of the same cell showing GFP-myosin-IB enriched at the border of the cup close to the plasma membrane. C, uptake of a particle by a cell expressing GFP-Arp3, indicating coincident localization of the Arp2/3 complex and the labeled actin. D, the phagocytic cup in a cell expressing GFP-coronin shows coronin remote from the membrane at the interface between the actin layer and the cytoplasmic space. The progressing edge of the cup is free of coronin. Bar, 10 μm.