Figure 4
From: Self-organizing actin waves that simulate phagocytic cup structures
Reversible suppression of actin wave formation by the PI3-kinase inhibitor LY-294002. Cells recovering from LatA treatment were allowed to form waves before 50 μM LY-294002 were added. In (A to D) time is indicated in seconds before and after addition of the inhibitor (t = 0), in (E) before and after its removal. In (A, B, and E) the LimEΔ-GFP label for actin (green) is superimposed on phase-contrast images showing cell shape (red). In (C and D) cells are double-labeled with mRFP-LimEΔ for actin (red) and GFP-clathrin light chains for clathrin-coated structures (green). A, B, actin wave formation is suppressed by LY-294002 while small actin patches are persisting. C, co-localization of the actin and clathrin labels in six patches at the substrate-attached surface of an LY-treated cell. D, clathrin and actin dynamics in an LY-treated cell. Arrowheads point to clathrin-coated structures (44 s) that recruit actin thus turning from green to red (49 s) and subsequently disappear from the membrane (55 s). E, recovery of wave formation in a big cell after the removal of Ly-294002. Bars, 10 μm in (A, B, and E); 5 μm in (C and D).