Figure 8

Simulation of tomographic reconstruction for randomly oriented filaments. Analysis of filaments (red) at high (A) and low (B) concentrations viewed over the IMC (yellow). Filaments of three different mean lengths were generated: 24, 48 and 72 nm (left column) and affected by low (middle column) and high (right column) noise; the tilting was performed around the parasite long axis (horizontal), the direction of the simulated electron beam was vertical. Note that longer filaments can be more reliably distinguished at lower noise (B, bottom row, central panel).