Different orientations of in silico simulated filaments. Left column: filaments with an average length of 48 nm are oriented in the direction of the electron beam and perpendicular to the parasite long axis corresponding to model I in figure 6. Middle column: filaments with an average length of 48 nm are oriented perpendicular to the electron beam and parallel to the parasite long axis, corresponding to model II in figure 6. Right column: filaments of 30 nm length connect the IMC and the plasma membrane (blue) and are viewed perpendicular to both the parasite long axis and the electron beam, corresponding to model III in figure 6. The noise in simulated tomograms increases from top to bottom. Note that filaments can be seen if oriented perpendicular to the parasite long axis, which is the tilt axis.