Figure 1

Data acquisition of FRET. Osteosarcoma cells U-2OS were transfected with plasmids expressing the donor molecule ECFP-HIF-1α and the acceptor EYFP-HIF-1β. 48 h later cells were scanned for fluorescence and FRET was measured. Data for the panels in the upper row were recorded for panel DD by donor excitation- donor detection, for panel DA by donor excitation- acceptor detection and in panel AA by acceptor excitation-acceptor detection.