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Figure 6 | PMC Biophysics

Figure 6

From: Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms

Figure 6

The hVDAC isoforms exhibit different degrees of colocalization with hexokinase-I. Quantitative colocalization analysis of any of the three hVDAC isoforms with hexokinase-I based on two-color STED images. Shown are the Pearson's correlation coefficients rp (A), the overlap coefficient r (B) as well as the colocalization coefficients M1 and M2 (C, D). As positive controls for full colocalization, we analyzed cells doubly labeled for hexokinase-I as well as cells co-expressing hVDAC1-Flag and hVDAC1-V5. Error bars: Standard deviation. The numbers on the columns represent the number of analyzed cells. The detailed values for all columns are given in Additional file 4. M1 and M2 may overestimate the level of colocalization because of the required thresholding. To demonstrate this effect, an intensity profile through a two-color STED image, where the cell was labeled for hVDAC2 and hexokinase-I, is shown (E). The fluorescence intensity was determined at the indicated line in the images. For details see main text.

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