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Figure 5 | PMC Biophysics

Figure 5

From: Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms

Figure 5

Two-color STED microscopy of the three hVDAC isoforms and hexokinase-I reveals individual protein clusters. U2OS cells expressing one of the three Flag-tagged hVDAC isoforms were labeled with antibodies directed against the Flag-tag and hexokinase-I. With confocal microscopy, neither individual hVDAC3 nor individual hexokinase-I clusters could be resolved (bottom row). In contrast, the corresponding STED images clearly resolved hVDAC3 and hexokinase-I clusters (third row). Two-color STED images of hVDAC1 or hVDAC2, imaged together with hexokinase-I, further shows that the hVDAC1/hVDAC2 domains are frequently composed out of smaller clusters. In the overlay the green color corresponds to the hVDAC signal and the red color to the hexokinase-I signal.

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PMC Biophysics

ISSN: 1757-5036