Figure 4

Kinetic model for molecule exchange at PML nuclear bodies. (A) Molecules with the potential to interact with PML nuclear bodies move by diffusion in the nucleoplasm outside nuclear bodies. Upon stochastic encounter, molecules associate and dissociate from the periphery of the nuclear body (k_on and k_off, respectively) and penetrate into and out of the shell of the nuclear body (k_in and k_out, respectively). (B-O) Fitting of the measured FRAP curves (blue dotted line) with the binding-diffusion model shown in (A) results in good fits (red lines).