Figure 3

Quantitation of FRAP experiments. FRAP experiments as described in Figure 2 were performed for all GFP-PML isoforms in PML positive (black curves) and PML negative cells (red curves) as indicated. A second mobility population of PML negative cells was observed for GFP-PML II and IV (blue curves). The graphs show the mean values from at least 20 FRAP evaluations as relative fluorescence intensity (RFI) after normalization to prebleach levels. Standart deviations (not shown) ranged between 10 to 15 percent of the mean values.