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Figure 8 | PMC Biophysics

Figure 8

From: Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayers

Figure 8

Application to cultured cells. Steady-state anisotropy, emission intensity, and peak ratio were assayed for S49 lymphoma cells both prior to and following treatment with ionomycin (300 nM). Panel A: Anisotropy was assayed at multiple wavelengths as described for Figure 1. Both the effects of ionomycin (triangles) and wavelength were statistically significant by two-way analysis of variance (p < 0.006 in both cases, n = 9, squares represent the untreated controls). The effect of ionomycin treatment on emission intensity at 585 nm was also significant (Panel B, p < 0.0001 by paired t-test, n = 8). The peak ratios (Panel C) were indistinguishable (p = 0.33 by paired t-test, n = 8) before and after ionomycin treatment. Error bars represent the SEM.

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PMC Biophysics

ISSN: 1757-5036