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Figure 7 | PMC Biophysics

Figure 7

From: Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayers

Figure 7

Temperature and cholesterol phase maps. The experiments of Figure 6 (equilibrated vesicles) were repeated at multiple cholesterol concentrations between 0 and 40 mol%. Color phase maps were generated at temperature and cholesterol resolution of 3°C and 5 mol% as explained in Materials and Methods. Intensity and ratio maps (red and blue, respectively) were created from the normalized values of the measurements with the brightest color representing a normalized value of 1.0. The anisotropy map (green) was created from normalized anisotropy values with the brightest color representing a normalized value of 0.0. The three maps were then overlaid to create a three-color composite map of the data (bottom panel).

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PMC Biophysics

ISSN: 1757-5036