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Figure 5 | PMC Biophysics

Figure 5

From: Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayers

Figure 5

Detailed emission peak ratio temperature profile. Emission intensities (at 585 and 621 nm) were collected in single degree increments from 20 to 50°C for refrigerated vesicles (solid circles), and the ratio of these intensities (585 nm/621 nm) was calculated. Error bars represent the SEM for four vesicle samples. The open circles denote a sample that was equilibrated at 50°C prior to cooling the sample to 25°C and then assaying the emission intensity at single degree intervals back up to 50°C. Data were normalized and scaled to the maximum ratio (set to 1.0) and the ratio of refrigerated samples at 25°C (set to 0.0).

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PMC Biophysics

ISSN: 1757-5036