Figure 4From: Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayersEmission intensity spectra for refrigerated and equilibrated vesicles. Emission spectra were collected at 25 (thin solid curve), 30 (dotted), 35 (dashed), and 45°C (heavy solid). Panel A: Vesicles were refrigerated for at least 24 h prior to fluorescence measurements. The inset displays the details of the 25 and 30°C spectra. Panel B: Vesicles were equilibrated at 50°C prior to adjusting to the experimental temperatures used in Panel A.Back to article page