Figure 3From: Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayersDetailed emission intensity temperature profile. Emission intensity (at 585 nm) was collected in single degree increments from 20 to 50°C for vesicles that had been stored at 4°C (solid circles). Error bars represent the SEM for five vesicle samples. The open circles denote a sample that was equilibrated at 50°C prior to cooling the sample to 25°C and then assaying the emission intensity at single degree intervals back up to 50°C. Data were normalized and scaled to the maximum intensity (set to 1.0) and the intensity of refrigerated samples at 25°C (set to 0.0).Back to article page