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Figure 1 | PMC Biophysics

Figure 1

From: Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayers

Figure 1

MC540 steady-state anisotropy spectra at various temperatures. Panel A: Vesicles were stored at 4°C. Anisotropy was assayed at four temperatures 15 (green), 25 (black), 38 (red), and 46°C (blue). Following collection of anisotropy data at 46°C, the samples were cooled back to 25°C and anisotropy was re-measured (violet). Error bars represent the SEM (n = 3-7) vesicle samples. Panel B: Vesicles were stored at room temperature and anisotropy was assayed at 25 (black), 38 (red), and 46°C (blue). Panel C: The vesicles of Panel B were stored overnight at 4°C after the data in Panel B had been gathered. The anisotropy was then assayed again at 25 (black), 38 (red), and 46°C (blue). Error bars represent the range of two samples in panels B and C. The data at 15°C from Panel A were included in Panels B and C to facilitate comparisons.

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