Figure 3

The connection between TUHMAs and primary cilia indicated by double immunolabeling with antibodies against tubulins and nucleoporins. A) A TUHMA labelled with mAb 414 (red) and B) tubule-forming detyr-T (green) both located in the same confocal section close to the substratum of cell growth. C) overlay of A and B indicating the co-localization of both fluorescence signals. D) TUHMA labelled with mAb 414 (red; arrow) and E) detyr-T (green) forming two tubules positioned orthogonally to each other both located in the same confocal section close to the substratum of cell growth. F) Overlay of D and E indicating that just one of the detyr-T containing tubules co-localizes with the TUHMA in D. G) Co-localization of a tubular structure labelled with mAb 414 and detyr-T in an upper confocal section perhaps harbouring the axoneme. F) Cilia in an upper confocal section: Two short cilia (arrows), numbered 1 and 2, labelled with antibodies against α-tubulin (green) are associated with red fluorescent dots resulting from labelling with mAb 414 raised against nucleoporins. The insert displays cilium 2 in more detail. In contrast to cilia 1 and 2, two longer cilia in neighbouring cells (arrowheads) are free of labelling with mAb 414. Bars, 5 μm.