Figure 2

Actin waves at the interface of different organizational states in the cell cortex. On the two sides of a propagating actin wave, different actin structures are found. A cell of Dictyostelium discoideum has been labeled with mRFP-ARPC1, a subunit of the Arp2/3 complex (red), and GFP-cortexillin I (green). The Arp2/3 complex is responsible for the nucleation of branched actin filament structures, cortexillin causes actin filaments to bundle preferentially in anti-parallel direction. The cell has been imaged using TIRF microscopy. It is in an extensive wave-forming state during the recovery of actin polymerization from treatment with LatA. Time after the first frame is indicated in seconds. Bar 5 μm. Data from [23].