Figure 1

Actin waves at the border of PIP3-rich areas. Propagation of an actin wave (red) at the border of a PIP3-rich area (green) of the plasma membrane. A cell of Dictyostelium discoideum is spread on a glass surface and imaged using TIRF microscopy. During propagation of the actin wave, the cell shape remains almost unchanged; the border of the PIP3-depleted area is indicated in the first frame by a dotted line. The cell has been labeled using mRFP-LimEΔ for filamentous actin and PHcrac-GFP for PIP3. The cell is in an extensive wave-forming state during the recovery of actin polymerization from treatment with LatA. Time after the first frame is indicated in seconds. Bar 5 μm. Data from [22].