Effect of membrane MBCD treatment on the temperature dependence of light scatter intensity. The experiments of Figure 2 were repeated in samples from which membrane cholesterol had been depleted by MBCD (see Materials and Methods). Based on previous calculations , samples contained 46.8% (untreated, open circles), 40.7% (treated with 0.3 mM MBCD, solid circles), or 19.0% (treated with 1.0 mM MBCD, open triangles) membrane cholesterol. Data were analyzed as in Figure 2 and expressed as mean ± SEM (n = 3–4). The effects of MBCD concentration and temperature were both significant by two-way ANOVA (p < 0.0001 in both cases, p = 0.04 for interaction). Two additional concentrations of MBCD not shown in the figure (0.1 mM or 44.9% cholesterol and 0.6 mM or 33.0% cholesterol) were included in the analysis of variance.