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Table 2 Tm values of 7E15 variants

From: Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteins

Protein

Tm (°C)

 

EGTA

Ca2+

Tb3+

CD2

61 ± 1

61 ± 1

-

EEDDD

41 ± 1

51 ± 1

-

EEDDE

44 ± 2

56 ± 1

63 ± 2

EEDDN

52 ± 2

58 ± 2

54 ± 1

EEDDQ

56 ± 1

61 ± 1

60 ± 1

EENDN

61 ± 2

59 ± 2

59 ± 2

NENDN

60 ± 3

58 ± 2

58 ± 4

  1. Using far-UV CD all Tm values were obtained in 10 μM protein solution with 1 mM EGTA, 10 mM Ca2+, and 0.1 mM Tb3+, respectively. The buffer system is 10 mM Tris, 10 mM KCl, and pH 7.4.