Figure 6From: Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteinsElectrostatics calculations. (A) Correlation of observed Tm values of the apo-7E15 variants and calculated changes in folding energy. (B) Correlation of calculated (ΔΔGb) and experimental results for mutational effects on binding free energy. The error bars are for experimental measurements.Back to article page