Figure 5From: Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteinsThermal denaturation. The thermal denaturation of EEDDE in the presence of 1 mM EGTA (solid diamond), 10 mM Ca2+ (open circle), or 0.05 mM Tb3+ (solid triangle) in 10 mM Tris, 10 mM KCl. The solid lines are generated by fitting the data to Equation 1.Back to article page