Figure 1From: Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteinsModel structure of designed proteins. (A) Model structure of EEDDD (7E15) and the coordination design of the variants. (B) The surface potential of Ca2+-free EEDDE, calculated using DelPhi[45], shows the highly-charged Ca2+-binding location. The calculation used interior and exterior dielectric constants of 4 and 80, respectively.Back to article page