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Table 1 Composition of liposomes used in fluorescence experiments.

From: Resolution of complex fluorescence spectra of lipids and nicotinic acetylcholine receptor by multivariate analysis reveals protein-mediated effects on the receptor's immediate lipid microenvironment

 

Mol %

Sample No.:

1

2

3

4

5

6

7

8

9

10

PyPC

0

0

5

DOPA

50

20

20

60

50

40

30

20

10

0

DOPC

50

40

40

0

10

20

30

40

50

60

NBD-Chol

0

40

  1. Samples 1 and 2 are control liposomes; sample 3 corresponds to the AChR-containing liposomes, with a lipid to protein molar ratio of 500:1. Excluding PyPC (5 mol% extra), final lipid concentration was 40 μM for all samples, amounting to 100%. Except for sample 2, all other samples were assayed in duplicate.