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Figure 4 | PMC Biophysics

Figure 4

From: Resolution of complex fluorescence spectra of lipids and nicotinic acetylcholine receptor by multivariate analysis reveals protein-mediated effects on the receptor's immediate lipid microenvironment

Figure 4

Förster resonance energy transfer (FRET) from the AChR. Smoothened fluorescence emission spectrum of an AChR PyPC-containing sample at 25°C showing the areas (A1 and A2) used to evaluate FRET. The dotted line indicates the average boundary wavelength at which the absolute value of the first derivative of the spectrum passed through a minimum. Excitation wavelength = 290 nm. Inset: FRET from AChR to PyPC as a function of temperature. FRET was calculated according to [1-(A1/A2)]. Liposomes contained DOPA/DOPC/NBD-Chol at a molar fraction 20/40/40 and a lipid to protein molar ratio of 500:1. PyPC concentration was 5 mol%. Error bars represent the S.D. of two independent samples.

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PMC Biophysics

ISSN: 1757-5036