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Figure 6 | PMC Biophysics

Figure 6

From: ATR-FTIR spectroscopy detects alterations induced by organotin(IV) carboxylates in MCF-7 cells at sub-cytotoxic/-genotoxic concentrations

Figure 6

Average IR spectra of MCF-7 cells with corresponding scores plots plotted on PCs 1,2 and 3 and loadings plots following 24-h exposure to 0.0001 μg Me3Sn(L3)/ml. Cells were seeded into 60-mm Petri dishes and allowed to attach for 24 h prior to exposure. Following exposure, cells were disaggregated with trypsin/EDTA solution, fixed in 70% EtOH, and applied to Low-E Microscope slides whereupon the suspensions were allowed to air-dry. A) 10 IR spectra were derived (vehicle control in blue, treated in red). B) From subsequent multivariate analysis, a 3-D scores plot on PCs selected to demonstrate best segregation of vehicle control (blue) vs. exposure (red) was constructed. C) Following cluster analysis, loadings plots were constructed on each relevant PC to identify the wavenumbers most responsible for segregation of clusters.

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