Figure 1

Effects of organotin(IV) carboxylates on per cent survival in MCF-7 cells. Cells (0.5 × 10³ cells) were seeded in 25 cm² flasks in the presence or absence of test agent and incubated for 24 h, as described in Materials and methods. Following addition of fresh medium, in the absence of exposure, cells were cultured undisturbed at 37°C and 5% CO₂ in a humidified atmosphere for 7 days. Surviving colonies were fixed and stained, and per cent survival was calculated by estimating the percentage of colonies counted over the number of cells initially seeded. Results are presented as the mean ± SD of the average values derived from triplicate measurements for each treatment condition in three independent experiments.