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  1. Research

    Combined use of steady-state fluorescence emission and anisotropy of merocyanine 540 to distinguish crystalline, gel, ripple, and liquid crystalline phases in dipalmitoylphosphatidylcholine bilayers

    The various lamellar phases of dipalmitoylphosphadtidylcholine bilayers with and without cholesterol were used to assess the versatility of the fluorescent probe merocyanine 540 through simultaneous measuremen...

    Hannabeth A Franchino, Brett C Johnson, Steven K Neeley, Rajeev B Tajhya, Mai P Vu, Heather A Wilson-Ashworth and John D Bell

    PMC Biophysics 2010 3:14

    Published on: 5 November 2010

  2. Letter

    Bistability in the actin cortex

    Multi-color fluorescence imaging experiments of wave forming Dictyostelium cells have revealed that actin waves separate two domains of the cell cortex that differ in their actin structure and phosphoinositide co...

    Carsten Beta

    PMC Biophysics 2010 3:12

    Published on: 24 June 2010

  3. Research article

    Combined molecular dynamics and continuum solvent studies of the pre-pore Cry4Aa trimer suggest its stability in solution and how it may form pore

    Cry4Aa toxin is one of the highly specific mosquito-larvicidal proteins produced by the bacterium Bacillus thuringiensis subspecies israelensis. It is thought to form pores in the larval midgut membrane that caus...

    Taveechai Taveecharoenkool, Chanan Angsuthanasombat and Chalermpol Kanchanawarin

    PMC Biophysics 2010 3:10

    Published on: 13 May 2010

  4. Research article

    Live cell flattening — traditional and novel approaches

    Eukaryotic cell flattening is valuable for improving microscopic observations, ranging from bright field (BF) to total internal reflection fluorescence (TIRF) microscopy. Fundamental processes, such as mitosis...

    Christian Westendorf, Albert J Bae, Christoph Erlenkamper, Edouard Galland, Carl Franck, Eberhard Bodenschatz and Carsten Beta

    PMC Biophysics 2010 3:9

    Published on: 19 April 2010

  5. Research article

    Assembly dynamics of PML nuclear bodies in living cells

    The mammalian cell nucleus contains a variety of organelles or nuclear bodies which contribute to key nuclear functions. Promyelocytic leukemia nuclear bodies (PML NBs) are involved in the regulation of apopto...

    Peter Brand, Thorsten Lenser and Peter Hemmerich

    PMC Biophysics 2010 3:3

    Published on: 5 March 2010

  6. Research article

    Two-color STED microscopy reveals different degrees of colocalization between hexokinase-I and the three human VDAC isoforms

    The voltage-dependent anion channel (VDAC, also known as mitochondrial porin) is the major transport channel mediating the transport of metabolites, including ATP, across the mitochondrial outer membrane. Bioc...

    Daniel Neumann, Johanna Bückers, Lars Kastrup, Stefan W Hell and Stefan Jakobs

    PMC Biophysics 2010 3:4

    Published on: 5 March 2010

  7. Research article

    Inverse tuning of metal binding affinity and protein stability by altering charged coordination residues in designed calcium binding proteins

    Ca2+ binding proteins are essential for regulating the role of Ca2+ in cell signaling and maintaining Ca2+ homeostasis. Negatively charged residues such as Asp and Glu are often found in Ca2+ binding proteins and...

    Anna Wilkins Maniccia, Wei Yang, Julian A Johnson, Shunyi Li, Harianto Tjong, Huan-Xiang Zhou, Lev A Shaket and Jenny J Yang

    PMC Biophysics 2009 2:11

    Published on: 21 December 2009

  8. Research article

    Simple modeling of FtsZ polymers on flat and curved surfaces: correlation with experimental in vitro observations

    FtsZ is a GTPase that assembles at midcell into a dynamic ring that constricts the membrane to induce cell division in the majority of bacteria, in many archea and several organelles. In vitro, FtsZ polymerize...

    Alfonso Paez, Pablo Mateos-Gil, Ines Hörger, Jesús Mingorance, Germán Rivas, Miguel Vicente, Marisela Vélez and Pedro Tarazona

    PMC Biophysics 2009 2:8

    Published on: 22 October 2009

  9. Research article

    The influence of membrane physical properties on microvesicle release in human erythrocytes

    Exposure of human erythrocytes to elevated intracellular calcium causes fragments of the cell membrane to be shed as microvesicles. This study tested the hypothesis that microvesicle release depends on microsc...

    Laurie J Gonzalez, Elizabeth Gibbons, Rachel W Bailey, Jeremy Fairbourn, Thaothanh Nguyen, Samantha K Smith, Katrina B Best, Jennifer Nelson, Allan M Judd and John D Bell

    PMC Biophysics 2009 2:7

    Published on: 24 August 2009

  10. Research article

    Three-dimensional studies of pathogenic peptides from the c-terminal of Trypanosoma cruziribosomal P proteins and their interaction with a monoclonal antibody structural model

    The acidic C-terminal peptides from Trypanosoma cruzi ribosomal P proteins are the major target of the antibody response in patients suffering Chagas chronic heart disease. It has been proposed that the disease i...

    Osvaldo A Martín, Myriam E Villegas and Carlos F Aguilar

    PMC Biophysics 2009 2:4

    Published on: 27 May 2009

  11. Mini-review

    The multiple faces of self-assembled lipidic systems

    Lipids, the building blocks of cells, common to every living organisms, have the propensity to self-assemble into well-defined structures over short and long-range spatial scales. The driving forces have their...

    Guillaume Tresset

    PMC Biophysics 2009 2:3

    Published on: 17 April 2009

  12. Research article

    An effective all-atom potential for proteins

    We describe and test an implicit solvent all-atom potential for simulations of protein folding and aggregation. The potential is developed through studies of structural and thermodynamic properties of 17 pepti...

    Anders Irbäck, Simon Mitternacht and Sandipan Mohanty

    PMC Biophysics 2009 2:2

    Published on: 8 April 2009

  13. Research article

    Membrane protein dynamics: limited lipid control

    Correlation of lipid disorder with membrane protein dynamics has been studied with infrared spectroscopy, by combining data characterizing lipid phase, protein structure and, via hydrogen-deuterium (H/D) excha...

    Balázs Szalontai

    PMC Biophysics 2009 2:1

    Published on: 6 February 2009

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