Figure 5
From: Assembly dynamics of PML nuclear bodies in living cells
PML SUMOylation sites K160 and K490 are required for PML body formation. The upper part shows a FRAP experiment of GFP-tagged mutant PML IV (K160/490R) in 3T3-PML-/- cells. Fluorescence was bleached in a circled area within the nucleoplasm (red circle). Confocal images were taken before (pre) and after (post) the bleach pulse and at different time points thereafter. Bottom part: FRAP experiments of GFP-tagged PML IV (K160/490R) were performed in 3T3-PML+/+ (black) and 3T3-PML-/- cells (red) and quantified. FRAP curves show mean values of at least ten measurements.